Journal: Biomolecules
Article Title: Biophysical and Solution Structure Analysis of Critical Residues Involved in the Interaction between the PupB N-Terminal Signaling Domain and PupR C-Terminal Cell Surface Signaling Domain from Pseudomonas capeferrum
doi: 10.3390/biom14091108
Figure Lengend Snippet: Global analysis of ITC isotherms for ( A ) PupB NTSD Q69K or ( B ) PupB NTSD H72D titrated into MBP-PupR CCSSD. The heats of binding (top panel), the isotherms with the curves for the global model (middle panel), and residuals of the global model fit (bottom panel) for the triplicate experiments are shown in black, gray, and light gray.
Article Snippet: ITC was performed using a Low Volume Nano ITC (TA Instruments, New Castle, DE, USA).
Techniques: Binding Assay